This study examines the ultrasonic disintegration of microalgae cells using a cascade emission plant, focusing on Chlorella cultures. A computer simulation was conducted to model pressure distribution, shear stresses, and cavitation effects within the device. The simulation results revealed maximum acoustic pressure of 3416 Pa and the highest surface deformation along the Y-axis at 2.863 × 10⁻⁹ m, confirming the validity of the device's operational frequency and design. Experimental research investigated the effects of ultrasonic power, irradiation time, and suspension concentration on cell disintegration. The highest cell reduction (68.24%) was observed at a 1:1 concentration and maximum power after 20 min, with significant reductions occurring within the first 3–5 min. At a 1:3 concentration, cell reduction reached 52.17%, demonstrating a concentration-dependent ultrasound efficiency. Experiments without liquid circulation indicated intensified cavitation, achieving 63.27% reduction at a 1:3 concentration. These findings highlight the effectiveness of ultrasonic technology in enhancing microalgae disintegration for biofuel and lipid extraction, providing a foundation for optimizing ultrasonic systems in industrial applications.