Differential absorption lidar (DIAL) is an effective way to measure atmospheric ozone with high accuracy. In order to select the appropriate differential absorption wavelength pairs to be used in DIAL to measure atmospheric ozone, a simulation experiment on wavelength selection was carried out. In the wavelength band of 250–750 nm, wavelength combinations with wavelength differences ranging from 1 to 50 nm were tested at 1 nm intervals for each wavelength, and the comprehensive error of each wavelength combination for DIAL measurement of atmospheric ozone was calculated through the simulation experiment. The feasibility and advantages and disadvantages of different wavelength combinations in the band for ozone measurement are comprehensively evaluated, and then the wavelength selection suggestions for different measurement needs are given based on the results of the simulation experiment.