Extremophilic microbes thrive in extreme environmental conditions and are an important resource for industries, especially in enzyme, metabolite production, etc. Extreme conditions of temperature, salinity, pressure, etc., led to the emergence of extremophiles with enzymes designed to survive in these hostile conditions. Extremophiles have evolved enzymes with remarkable structural stability and catalytic activity in extreme environments through adaptive evolution, which presents potential opportunities for biocatalysis and enzyme mechanistic studies. There are various significant aspects associated with extremozyme extraction, fermentation condition optimization, isolation, and purification methods, such as ultrafiltration and chromatography for producing high-purity enzyme preparations, which are prerequisites for efficient industrial use. The current article discusses the potential of enzymes and downstream processing targeted at improving functionality from extremophilic microorganisms.