This study focuses on the development and characterisation of a novel hybrid bioink based on natural and synthetic hydrogels for application in 3D bioprinting. The main objective is to evaluate the potential of these materials for regenerative medicine by optimising their composition and assessing their mechanical, rheological, and printability properties. The ultimate goal is to identify the most suitable formulation for fabricating biomimetic three-dimensional structures. Hydrogels were synthesised using gelatin methacrylate (GelMA) and konjac, with lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as a photoinitiator. Rheological characterisation was performed via rotational rheometry, while printability and self-supporting capacity were assessed using a BIO X 3D bioprinter. Mechanical performance was evaluated through flow strength and Young’s modulus testing. The resulting hybrid hydrogels demonstrated suitable printability and structural stability. The incorporation of konjac into GelMA formulations enhanced mechanical performance and structural support. Rheological analyses indicated pseudoplastic behaviour with controlled viscosity, facilitating accurate layer-by-layer deposition without collapse. Hydrogels with higher GelMA content exhibited greater mechanical strength, whereas those with higher konjac content showed increased elasticity and better adaptation to biological conditions. The development of GelMA-KGM hybrid hydrogels represents a promising strategy for 3D bioprinting, providing improved mechanical integrity, printability, and biocompatibility. The optimised formulation enables the fabrication of stable and adaptable 3D constructs suitable for applications in regenerative medicine. Further studies are recommended to explore their integration into functional tissue models and their potential use in advanced therapeutic strategies.