Ensuring a sterile environment in operation theatres is paramount for infection prevention and patient safety. This paper presents an automated sterilization system combining UV-C radiation and hydrogen peroxide vapor to achieve efficient and thorough disinfection. The proposed system integrates germicidal UV-C lamps to neutralize airborne microorganisms and an ultrasonic mist generator to disperse hydrogen peroxide vapor, reaching inaccessible surfaces and enhancing sterilization coverage. Safety is prioritized through real-time motion detection sensors that halt the process upon human presence, preventing accidental exposure. A programmable microcontroller supervises the entire cycle, offering reliability and ease of use. Future advancements include IoT-enabled features for remote monitoring, data logging, and predictive maintenance. This solution addresses limitations of traditional methods, streamlining hospital sterilization protocols while ensuring safety, efficiency, and adaptability to modern healthcare demands.