An interesting and ongoing area of research is the gender effect, which examines how gender, as a social and structural variable, affects the complex interplay with information systems. Undoubtedly, mobile applications are the means of delivering information, communication and entertainment, becoming an inseparable part of today’s society, regardless of gender. However, despite the priority given to understanding and meeting user needs, a wide range of usability issues are still being reported. On the other hand, there is still a paucity of research that has made an effort to investigate how gender affects users’ perceived usability of mobile applications. This study aims to fill this gap by examining the gender effect on the set of 36 apps through a survey conducted on a sample of 838 users. Several interesting findings became evident in the course of the study. First, the perceived importance of using mobile applications in the workplace and in personal life does not differ between men and women. Second, on average, women reported experiencing more usability problems with mobile applications than men. Third, perceived usability appears to align with app popularity within the sample. Fourth, less than 42 percent (15 out of 36) of mobile application usability was rated higher among men. Fifth, gender influences the perceived usability of about 22 percent (8 out of 36) of the mobile applications, with only one rated higher by men.