Leukemias are hematological malignancies characterized by the accelerated production of abnormal leukocytes that interfere with the production of other blood cells in the bone marrow. Rapid and accurate diagnosis is essential in these diseases. The aim of this study was to examine the capabilities of the Sysmex XN 3100 analyzer's digital microscopy in differentiating blasts and immature granulocytes in patients with leukemia, and to compare the results with manual microscopy, which is considered the gold standard according to CLSI guidelines. 31 whole blood samples from patients diagnosed with leukemia were analyzed using the Sysmex XN 3100 analyzer's digital microscopy method and manual optical microscopy. The results were collected and statistically processed through Passing-Bablok regression and Bland-Altman plot analysis. Comparative analysis showed proportional differences in blasts and immature granulocytes when comparing the primary data of digital microscopy with manual, while reclassifying them proportional difference between the methods is lost. A systematic difference between the methods was recorded but not statistically significant. Bland-Altman analysis also shows that the mean difference between the methods is not statistically significant and is further reduced by reclassification. The variability of the results is high, which in practice can cause larger deviations in the results in individual cases. In general, the obtained results show good performance of digital microscopy, especially secondary data compared to manual microscopy, when it comes to the differentiation of blasts and immature granulocytes in leukemia, which allows for greater application of digital microscopy in routine work.