This paper focuses on the design and development of a UV-C (Ultraviolet-C) -based device intended for room disinfection. UV-C radiation, with wavelengths between 200–280 nm, is well known for its germicidal properties, effectively inactivating bacteria, fungi, and viruses by disrupting their DNA and RNA. This paper aims to create a practical and efficient solution for reducing microbial contamination in enclosed spaces, particularly in the context of increased hygiene demands due to global health concerns. The device was built using commercially available UV-C lamps, an ozone generator, and an electronic control system for timed operation in the absence of direct human intervention. The device is intended to be a low-cost solution available to anyone who needs this solution. Some tests were conducted in order to see the efficacy of the device. This paper highlights the potential of UV-C technology as a non-chemical, environmentally friendly method for disinfection. Due to the impact of the COVID-19 pandemic, there are many users of this type of disinfection but also, it is intended to identify the situations in which this type of disinfection is not used appropriately, affecting either people around these systems or not being efficiently used. The final device can be adapted for various indoor environments such as hospitals, offices, classrooms, and homes.