Microfluidic devices enable the controlled analysis of cells, supporting studies in cell behavior, drug screening, and disease modeling. These systems are fabricated from various materials, including polymers such as PMMA and PDMS, as well as glass, silicon, and paper. The selection of substrate depends on the application, considering cost, biocompatibility, chemical resistance, and optical properties. However, the development of low-cost, biocompatible materials for microfluidic cell culture platforms remains a key challenge in biomedical engineering. In this study, we evaluate the biological compatibility of a commercially available UV-curable nail sculpting gel for potential use in microfluidic devices. We assess cytotoxicity, cell adhesion, proliferation, and migration across five human cell lines (A549, ARPE-19, HT-29, HT-1080, and 293T). Indirect cytotoxicity tests (ISO 10993-5) revealed no significant reduction in viability. All cell lines adhered successfully to the gel surface, although proliferation rates varied among lineages, suggesting surface-dependent effects. Wound healing assays confirmed migration capability, with HT-1080 and ARPE-19 cells exhibiting the fastest closure rates. Our results demonstrate that UV nail gel supports key cellular functions, offering a promising low-cost alternative for organ-on-chip and microphysiological system applications.