This chapter explores the resistance of vivisected dogs in Victorian laboratories. It shows how these animals’ physical and emotional responses challenged Claude Bernard’s mechanistic view of science, as the dogs’ embodied resistance disrupts the ideals of neutrality and objectivity in scientific practice. The chapter also reveals gendered and imperial narratives woven into experimental physiology, showing how women and animals were seen as suitable subjects for scientific study. The story reaches its peak with the dramatic Brown Dog Affair of 1903—a historic protest in London that turned canine suffering into a rallying point for working-class activists, feminists, and animal rights advocates. Their shared outrage over the dog’s suffering sparked ethical debates, pushing society to confront issues of empathy, morality, and the principles of scientific knowledge.