Picotechnology is an emerging discipline that manipulates matter at the sub-nanometer scale, especially within the region of 10−12 m. Picotechnology utilizes the unique physical, chemical, mechanical, and optical features of materials found at the picoscale, rather than just operating at smaller dimensions. Visible particle sizes of solid matter are similar to those observed in a normal optical microscope. Picoscale allows scientists to create novel processes that improve biological systems, medicine, imaging, computing, printing, chemical catalysis, and materials synthesis, among other domains. These features have the potential to drive efficiency, sustainability, and environmental friendliness across different industries. This chapter emphasizes the potential for Picotechnology to revolutionize biomedical imaging, biosensing, and drug delivery, offering deeper insights into the basic principles. The combination of Picotechnology and existing biological procedures has the potential to open up new frontiers in diagnostics, medicines, and biotechnology, pushing the limits of molecular biology and medical research.