This work focuses on the implementation of 3D printing molds for manufacturing Lab-on-a-Chip devices. Due to its low-cost materials compared to traditional methods, such as photolithography, the 3D printing method is a viable alternative for creating microchannels; however, it has its limitations. These are explored in this research, and the detailed manufacturing method for obtaining a Lab-on-a-chip with polydimethylsiloxane (PDMS) material is explained. PDMS is a biocompatible material used primarily due to its properties. The primary issue with standard methods, such as lithography, is the time-consuming design of the mold and all the elements required to manufacture the Lab-on-a-chip, despite the high costs associated with the necessary machinery.