In this chapter, we present three models of heterogeneous non-rotational symmetric apertures. The first aperture has a uniform half-circle, and the other has a linear function, while the second aperture has a uniform half-circle combined with a quadratic function for the other half of the circle. The third model of aperture consists of half a linear and half a Hamming function. The Point Spread Function (PSF) is computed for all apertures and compared with circular apertures. The PSF for the rotation of heterogeneous apertures is deduced. The object of this study is the computation of the resolution from the PSF for these heterogeneous apertures and its comparison with the case of uniform circular apertures. Finally, the autocorrelation of the apertures under investigation, which represents the Coherent Transfer Function (CTF) in the Confocal Laser Scanning Microscope (CLSM), is computed. All images obtained make use of MATLAB code.