In the context of transport networks, synchronization techniques refer to a set of optimization approaches whose output is the timetables of different lines obtained through the minimization of transfer waiting time and vehicle bunching. Accordingly, synchronization techniques can improve the overall quality of transfers in transport systems by making them smoother for passengers. The synchronization of transport lines at strategic locations improves the system’s overall connectivity and integration and, ultimately, its attractiveness to passengers. This work aimed to synchronize strategic transport lines connecting Porto to other cities, having Porto as the only focal point. The morning peak hours were selected as the analysis period. A synchronization model already proposed in the literature was adapted and applied to this case study. The results include the comparison between the real and synchronized timetables and the sensitivity analysis of the solutions obtained by changing headway and time window parameters.