Bamboo is a naturally Cultivating and sustainable material, has proven to be a good alternative to reinforcement and fibers in construction applications. It develops excellent compressive strength (CS) and tensile strength (TS), making it more economical reinforcement material in concrete applications. In this research work, four different types of bamboos Dendrocalamus strictus and D-strictus, Assam and Bengal were sourced from four different states in India, to conduct a comparative analysis. The work objective to evaluate their behavior of bamboo under different loading and weathering conditions. The physical and mechanical properties of four bamboo types were tested, including moisture content and shrinkage. The compressive strength, split tensile strength, and shear strength (SS) tests were conducted following by Indian Standard (IS) guidelines. A bond test was also conducted to determine the adhesion between bamboo and concrete. The results conclude Dendrocalamus strictus produced superior resistance to shrinkage and moisture compared to remaining three bamboos. The mechanical properties, Dendrocalamus strictus achieved highest CS of 49.02 MPa. A similar trend was observed in TS strength tests. For SS, Dendrocalamus strictus demonstrated 40.59 MPa, significantly higher than the 30.11 MPa develop by D-strictus. The superior physical and mechanical properties, Dendrocalamus strictus was further evaluated for its bonding behavior with M30 grade concrete. The bond strength of untreated bamboo was found to be 0.2 N/mm2. This study highlights the potential use of Dendrocalamus strictus as a strong and sustainable reinforcement material in concrete applications.