Tissue engineering and regenerative medicine (TERM) offer immense potential for treating organ failure, traumatic injury, and multiple diseases like cancer. Significant breakthroughs are upcoming in key areas such as bioprinting, biomaterial innovation, stem cell therapies, immunoengineering, organ-on-a-chip models, artificial intelligence, or gene editing. Nevertheless, important challenges must be addressed to realize the full potential of TERM, which include vascularization and innervation of large constructs, immune rejection, ethics, interdisciplinary collaboration, and standardization and regulation. This last chapter discusses the areas where pivotal breakthroughs for TERM are envisaged and ends with a brief reminder and discussion of the challenges still to overcome for advancing the field towards clinical translation.