Hashimoto’s disease or Hashimoto’s thyroiditis is an autoimmune disorder of the thyroid gland. The pathophysiology of the disorder is characterized by an increased volume of the thyroid gland, infiltration of the parenchyma by lymphocytes, and the presence of antibodies specific for thyroid antigens. The diagnosis of Hashimoto thyroiditis is based on the presence of clinical symptoms, anti-thyroid antibodies, and certain histologic features. The present study aimed to collect biomarkers and create a web-based application for detecting the disease through machine learning. The web application was created using Python and Random Forest algorithm was selected to train the existing dataset. Taking advantage of machine learning algorithms, the application provides users with a practical means for predicting immortality based on their blood tests. Although there are limitations that need to be addressed, representing a valuable tool for early diagnosis and management of the disease is required.