Nanocomposites synthesized through biological routes have great significance in recent biomedical applications. Besides, metal nanocomposites (MNCs) are gaining importance nowadays due to their promising physicochemical features. Biogenic MNCs production is an inexpensive alternative that uses plants, animals, fungi, and bacteria. Though different approaches are commonly used in the preparation of MNCs, the selection of approach is based on the size, morphology, and crystal structure of NCs. MNCs are generally employed in the cancer diagnosis and treatment, targeted drug delivery, and radio imaging. Accordingly, in this chapter, the methods of MNCs synthesis, biological routes of MNCs synthesis such as using plants, microbes, and other biomaterials, their physicochemical characterization, and their potential biomedical applications have been discussed.