The growing human population and their lifestyle demand urbanization and industrialization that indeed affect the global environment directly or indirectly. Radionucleotide wastes generated from various natural and anthropogenic sources can have significant impact on human health and ecosystem. These contaminants if not managed properly can pollute soil, water bodies, and air, posing detrimental effects on living organisms. Exposure to these radionuclides may result in conditions such as anaemia, haemorrhage, thyroid tumours, skin cancer, neurological and reproductive complications. Various remediation techniques have been employed to treat these radioactive wastes, of which the most common are chemical precipitation, ion exchange sorption, acid digestion, and electrochemical oxidation. These traditional methods often damage the environment and lead to secondary pollution. So there exists a demand of bioremediation, a process that degrade and detoxify waste for a permanent remediation without damaging the ecosystem, which is possible by utilizing biological means. The chapter here introduces the innovative use of fungi in addressing the challenges posed by radioactive wastewater by employing fungal properties like biosorption, bioaccumulation, and biofilm formation to bioremediate contaminated environments by absorbing, and metabolizing heavy metals and radioactive isotopes. Mycoremediation is more economical, sustainable and more ecofriendly as it creates least or no secondary pollution. Synergistic techniques like myconanotechnology, fusion of mycoremediation with phytoremediation, and application of genetically engineered fungi could make the remediation process more promising and more efficient over the conventional methods.