Water pollution is on the increase because of human population and activities increases, unmanageable agronomic practices and rapid development of industries, and it is a main worldwide concern. The subsequent mixture of liquid and waste is known as leachate. It is an enormously contaminated wastewater, threatens surface, ground waters, and the surrounding soil and needs acceptable management before release. Leachate, a harmful contaminant that effects on human health, cleanliness, and aquatic life, is a liquid produced from the bottommost of solid waste removal facilities. Leachate is processed through three methods such as chemical, physical and biological treatments. Among, leachate treatment methods, the biological methods are more ecologically benign and low cost than the physiochemical management approaches. The usage of naturally occurring microbes like fungi, bacteria, or plants to control contaminated wastewater has confirmed to be active and efficient. Over the previous few years, usage of fungal based management methods have developed prevalent because of their capability to synthesize oxidative and degrading enzymes such as lignin peroxidases, laccases, and manganese peroxidases. Fungi have revealed enhanced elimination efficacy in terms of ammonia, dye, and toxicity in the form of COD than the conventional leachate management. Fungi, with their outstanding mechanisms of degradation of recalcitrant toxic compounds by biotransformation, immobilization, and biosorption, have been applied to control leachate. The chapter contains various enzymes involved in the fungal degradation of pollutants and the oxidative and degrading enzyme mechanisms for control of leachate. The effect of factors such as temperature, pH, dosage difference, contact period, heavy metals and ammonia are deliberated.