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Analyzing the published data and the novel methods for monitoring compounds such as drugs, hormones, toxins, small molecules, proteins, and pathogens, it is evident that there is a considerable effort directed toward various detection principles. These include the use of labels and label-free methods, switching and displacement strategies, signal turn-on and turn-off moieties, as well as signal enhancementSignal enhancement and amplification techniques. Sensors, whether based on antibodies, aptamers, or phages, are often tailored to operate under specific conditions. In the following section, we will focus our discussion on the challenges associated with sensing platforms.

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Problems and Perspectives: Catalytic Versus Affinity Biosensing, Sensitivity, Selectivity and Specificity, Reproducibility, Calibration and Uncertainty, Regeneration, Signal Enhancement

  • 章节

  • 发表日期：

    2025年

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  • Aleksandr Simonian,
  • Mary Anitha Arugula,
  • Paolo Bollella

摘要

Analyzing the published data and the novel methods for monitoring compounds such as drugs, hormones, toxins, small molecules, proteins, and pathogens, it is evident that there is a considerable effort directed toward various detection principles. These include the use of labels and label-free methods, switching and displacement strategies, signal turn-on and turn-off moieties, as well as signal enhancementSignal enhancement and amplification techniques. Sensors, whether based on antibodies, aptamers, or phages, are often tailored to operate under specific conditions. In the following section, we will focus our discussion on the challenges associated with sensing platforms.