Mesotherapy is a minimally invasive treatment method used for both medical and cosmetic purposes. It allows for the direct application of medications to affected areas through intradermal injections. It is utilized in various conditions, including pain management, sports injuries, rheumatological diseases, chronic pain, vascular diseases, cellulite treatment, fat reduction, skin aging prevention, and hair loss. In cosmetic applications, it is a popular choice for anti-aging treatment, skin rejuvenation, and regional slimming. The substances used in mesotherapy include vasodilators, anti-inflammatory agents, vitamins, minerals, proteolytic enzymes, and plant extracts. The treatment is typically personalized, with the formulations and session frequency varying according to individual needs. Mesotherapy is preferred for skin rejuvenation, wrinkle reduction, and prevention of hair loss. In skin rejuvenation treatments, vitamins, minerals, hyaluronic acid, and amino acids are used to activate fibroblasts, enhancing collagen and elastin production. Side effects are generally mild, with temporary bleeding, bruising, and swelling at the injection site being common. However, in rare cases, subcutaneous nodule formation, hyperpigmentation, and localized allergic reactions may occur. In conclusion, while mesotherapy has gained popularity as a treatment method characterized by the use of low-dose products and minimal side effects, uncertainties remain regarding the efficacy of many substances and the standardization of treatment protocols.