In China, the volume of tobacco waste constitutes approximately 25% to 30% of the total cigarette production output. Bio-extraction technology, which is widely employed to transform tobacco waste into valuable resources, encompasses intricate heat and mass transfer processes. Accurately obtaining the thermophysical properties of tobacco is essential for studying the heat transfer phenomena within the tobacco waste bio-extraction process. In this study, five distinct brands of tobacco were selected to investigate the critical thermophysical properties of tobacco waste, including specific heat capacity and thermal conductivity. The specific heat capacity and thermal conductivity of tobacco within the temperature range of 40 to 120 ℃ were primarily found to be between 1.08 to 1.38 J/g·°C and 0.0594 to 0.0749 W/(m·K), respectively. Empirical formulas were derived to describe the temperature and moisture content dependence of the specific heat capacity and thermal conductivity of tobacco within the 40 to 120 ℃ range, with correlation coefficients exceeding 0.97383.