With all the progress in cancer biology and treatment, the pathophysiology of the cancer is still not thoroughly known, and its treatments are limited. The advent of tissue engineering and regenerative medicine has revolutionized the treatment of various disorders, including genetic diseases, neurological and neurodegenerative diseases, organ dysfunction, and cancers. Tissue engineering helps develop promising in vitro and in vivo models, such as spheroids, organoids, and organ-on-a-chip for studying cancer biology and evaluating the safety and effectiveness of novel antitumor drugs. Moreover, drug delivery systems that use scaffolds and stem cells enable sustained and localized release of antitumor drugs. Scaffolds and stem cells could also be utilized in cancer immunotherapy and healing the wounds caused by the resection of tumors. Advancements in tissue engineering and regenerative medicine may provide therapeutic approaches to treat tumors effectively. This chapter will discuss different aspects of tissue engineering and their application in developing novel therapeutic approaches for cancer treatment.