Given the lack of literature on incubators, this research focuses on the analysis of entrepreneurial motivational demand as a crucial element in the development of the entrepreneurial environment within a learning management model in incubators (MOGAI). The study uses a mixed descriptive-correlational approach, with four stages including systematic literature review, meta-analysis, principal component analysis and validation of the model with experts. The principal component analysis indicates that five variables (effort and vision, hard work, entrepreneurial training, work team and persistence) contribute significantly to the entrepreneurial motivational demand factor. The research not only provides a significant contribution to entrepreneurship issues by presenting a statistically proven model, but also offers an application input in the management of learning in incubators. This model, validated in the Colombian context, has the virtue of enriching the understanding of incubation projects, generating an impact in the academic and business sectors, as well as in public policies. It highlights the importance of the entrepreneurial motivational requirement in the development of the entrepreneurial spirit, contributing 15% of the MOGAI. This finding makes possible the importance of establishing the motivational requirement as a crucial factor in future research and in the adoption of incubation projects.