As the layout optimization edges of virtual simulation laboratory are mostly set as area optimization, the efficiency of layout processing is affected, which leads to the decrease of the final optimization ratio of area allocation. For this reason, a virtual simulation laboratory layout optimization method based on multi-input feature fusion is proposed. According to the current layout optimization requirements, the virtual simulation laboratory layout functional unit division, the laboratory overlap position adjustment, the use of multi-node approach to improve the layout processing efficiency, to achieve multi-node layout edge optimization. The virtual simulation laboratory layout optimization model with multi-input feature fusion is designed, and the laboratory layout hierarchy cross-simulation verification is adopted to realize layout optimization processing. The test results show that after applying the proposed method, the final optimized ratio of region allocation can reach below 8.5. The optimization method for the layout of the designed virtual simulation laboratory is more efficient and reliable.