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Advances in food technology demand efficient and precise analytical methods to ensure safety, quality, and regulatory compliance. Capillary electrophoresis (CE) has emerged as a powerful tool for food analysis, offering high-resolution separation of diverse components such as carbohydrates, proteins, lipids, phenolics, and contaminants within complex matrices. When coupled with mass spectrometry (CE-MS), it combines exceptional separation efficiency, minimal sample requirements, and rapid and efficient analysis of diverse food components such as carbohydrates, proteins, lipids, phenolics, and contaminants, outperforming traditional techniques like HPLC in resolving multicomponent mixtures with varying chemistries. CE also reduces the need for extensive sample cleanup while maintaining automation capabilities. Despite these advantages, its adoption in food analytics remains limited. This chapter explores CE’s fundamental principles, methodologies, and applications in food science, covering nutritional profiling, contaminant detection, and authenticity assessment. It critically evaluates CE’s performance against conventional methods, emphasizing its integration with advanced sample preparation, including online stacking and detection systems (such as MS LIF) to enhance sensitivity and specificity. It also discusses emerging innovations such as microchip CE and nanomaterial-enhanced capillaries, which are highlighted for their potential to revolutionize portable and high-throughput analysis. The discussion extends to future trends, including green chemistry approaches, automation, and compact analytical devices, positioning CE as a versatile solution for modern food industry challenges. It also compares separation modes such as zone electrophoresis, isoelectric, and micellar electrokinetic chromatography). The chapter guides practitioners in selecting optimal methods for specific needs. CE’s adaptability and efficiency underscore its potential to advance food safety, quality control, and traceability, bridging research and industrial application gaps.

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Capillary Electrophoresis (CE) for High Resolution Food Component Separation

  • 章节

  • 发表日期：

    2025年

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  • Aduloju Emmanuel Ibukun,
  • Omachi Bosede Alice,
  • Olawale Salaudeen Abdulwasiu,
  • Semail Nadhiratul-Farihin

摘要

Advances in food technology demand efficient and precise analytical methods to ensure safety, quality, and regulatory compliance. Capillary electrophoresis (CE) has emerged as a powerful tool for food analysis, offering high-resolution separation of diverse components such as carbohydrates, proteins, lipids, phenolics, and contaminants within complex matrices. When coupled with mass spectrometry (CE-MS), it combines exceptional separation efficiency, minimal sample requirements, and rapid and efficient analysis of diverse food components such as carbohydrates, proteins, lipids, phenolics, and contaminants, outperforming traditional techniques like HPLC in resolving multicomponent mixtures with varying chemistries. CE also reduces the need for extensive sample cleanup while maintaining automation capabilities. Despite these advantages, its adoption in food analytics remains limited. This chapter explores CE’s fundamental principles, methodologies, and applications in food science, covering nutritional profiling, contaminant detection, and authenticity assessment. It critically evaluates CE’s performance against conventional methods, emphasizing its integration with advanced sample preparation, including online stacking and detection systems (such as MS LIF) to enhance sensitivity and specificity. It also discusses emerging innovations such as microchip CE and nanomaterial-enhanced capillaries, which are highlighted for their potential to revolutionize portable and high-throughput analysis. The discussion extends to future trends, including green chemistry approaches, automation, and compact analytical devices, positioning CE as a versatile solution for modern food industry challenges. It also compares separation modes such as zone electrophoresis, isoelectric, and micellar electrokinetic chromatography). The chapter guides practitioners in selecting optimal methods for specific needs. CE’s adaptability and efficiency underscore its potential to advance food safety, quality control, and traceability, bridging research and industrial application gaps.