Wood may absorb or release water depending on the surrounding air conditions (relative humidity and temperature), impacting its mechanical properties, particularly stiffness and strength at room temperature. In the context of timber design, scant information is available on the effects of temperature, such as during exposure to fire. The current challenge involves understanding the residual properties of non-carbonized wood structural elements that are subjected to temperature up to 200 °C, to ensure occupant safety. Building standards indicate that the compressive strength of wood at 100 °C is 25% lower than at 20 °C, yet the role of wood moisture content is not explicitly addressed. Experiments conducted by CSTB in 2020 revealed that, within the 20–100 °C temperature range, moisture content significantly influences strength. This paper aims to better investigate how temperature and moisture content affect the mechanical properties of wood. The experiments entailed examining the response of glued laminated timber (GL24H) to diverse levels of moisture content and temperature. Consequently, several heat protocols were examined to assess the impact of temperature and moisture content on timber design. Specifically, P1 protocol served as the reference baseline at 20 °C for subsequent tests, while P1bis et P2 protocols were conducted at four temperatures (60, 100, 150 and 200 °C) with varying heating durations in an oven. Furthermore, the discussion of all results takes into account sample sizes.