Nanonutrients have special therapeutic potentials due to their small size and high surface-to-volume ratio. The study of efficacy and safety of new marketable nutraceutical formulations is essential in order to produce new marketable nutraceutical formulations. The diffraction limit reduces the resolution of current optical equipment, and improving on this was previously thought impossible. By microscopy, the structure, shape, and elemental proportion of nanoparticles can be visualized which makes modification of these structures easy. Microscopy also well describes the physical and chemical properties of the nanoparticles by characterizing them. Optical super-resolution imaging, which has seen significant expansion and increased global attention in recent years, will find applications in a variety of sectors, including nutraceutical and nanonutraceutical evaluation. The most important microscopic methods used are scanning electron microscopy, transmission electron microscopy, atomic force microscopy, scanning tunneling microscopy, confocal scanning microscopy, and super-resolution microscopy. This review describes microscopy and nanoscopy with its characteristic features and tools for microscopic evaluation of nutraceuticals and nanonutraceuticals.