Over the past few decades, there has been a global increase in the consumption of fresh fruits and vegetables, which has resulted in a rise in foodborne disease outbreaks linked to horticultural crops. Numerous pathogenic microbes, including viruses, bacteria, protozoa, and helminths, are responsible for significant foodborne outbreaks across the globe. Among the common pathogens that can be harmful to human health include Salmonella species, Listeria monocytogenes, Escherichia coli, Giardia lamblia, and Entamoeba histolytica, as well as hepatitis A, enteroviruses, echoviruses, rotaviruses, and Ascaris lumbricoides. All the historical episodes of disease outbreaks highlight the importance of microbial monitoring in the horticultural crops. However, the timely detection and monitoring of food borne pathogens in horticulture crops, can substantially reduce the risks involved through their consumption. Currently, there are numerous cultural and non-cultural methods for identifying them. Many new rapid technologies have emerged to overcome the drawbacks of conventional methods and provide accurate and timely results. Conventional approaches have several limitations, such as time-consuming detection and arduous procedures.