This paper presents an AI-enhanced virtual reality (VR) escape room designed to educate users about sustainable fashion practices, with a specific collaboration on the heritage of Scotland’s Harris Tweed Hebrides, a brand established in 1840. The project leveraged AI-driven technologies such as Polycam and Luma to scan 3D Harris Tweed garments, creating detailed models seamlessly integrated into a Unity-based VR escape room. Within the experience, players interact with AI-powered features, including OpenAI’s ChatGPT, which serves as a conversational agent to provide real-time information and answer user queries about sustainable fashion. The VR application, accessed via an Oculus Quest 2 headset, immerses users in a virtual house filled with interactive puzzles and challenges. To evaluate the effectiveness of the VR experience, a blind study was conducted, comparing the learning outcomes of participants who received the same educational content in a traditional printed format versus those who engaged with the immersive VR environment. The findings aim to demonstrate how interactive, game-based learning can enhance users’ understanding of sustainable fashion compared to traditional educational methods, ultimately fostering environmentally conscious consumer behavior.