Vitamin B6 (B6) is a very important water-soluble vitamin that plays a vital role in well over 100 different biochemical enzyme reactions. Vitamin B6 is found in a variety of animal- and plant-based foods as it is not produced in humans. It is a complex that encompasses six compounds with the active forms being pyridoxal 5′-phosphate (PLP) and pyridoxamine 5′-phosphate (PMP). The majority of Vitamin B6 is stored in muscle tissue and its deficiency is generally rare, occurring in the setting of nutritional deficiency and other vitamin or micronutrient deficiencies. We aim to provide a concise overview of Vitamin B6 metabolism, overall role in cellular and biochemical enzyme reactions, and deficiency and toxicity states.