The present research is entitled “MULTICRITERION ANALYSIS BASED ON GIS FOR THE POTENTIAL ZONING OF ARTISANAL TILAPIA AQUACULTURE (Oreochromis eng) IN GUAYAS, ECUADOR” and bases its application on the establishment of a significant potential to improve the productivity and sustainability of tilapia aquaculture by identifying suitable areas within the geographic and environmental factors through the use of the geographic information system (GIS) to represent and overlay the cartographic information. 1) The identification of geographic and environmental factors allowed to identify the best areas for aquaculture. Data were collected and categorized on natural and artificial water sources, temperatures (from 23 ℃ to 30 ℃), altitudes (from 0 to 1,200 m above sea level), slope levels from 0 to 7%, and exclusion zones that included protected areas, anthropogenic zones, and flood zones. 2) Representation of Cartographic Information, this was carried out through the GIS, which provided the elements required to implement a detailed spatial analysis, where the areas were classified as “Suitable”, “Moderately Suitable” and “Not Suitable”. On the other hand, the zoning criteria were integrated, which allowed the design of individual maps of each of the variables identified in advance, which facilitated the spatial visualization for the development of aquaculture in the province of Guayas. 3) Application of the Cartographic Information Overlay System, in this process multiple layers of geospatial data were combined, considering the suitability criteria for the determination of fish farming areas and performing a presence-absence check to corroborate the identified spatial data, which allowed the categorization and integration of the polygons, facilitating the identification of the most suitable areas (with a range of 0 to 6, where 6 is the most suitable area) for tilapia aquaculture in the province of Guayas. According to the overlay of geographic data, of the 15,338.06 km2 that make up the province of Guayas, 7,606.64 km2, or 49.28%, are suitable for tilapia aquaculture. Zones of geographic-environmental suitability and exclusion zones (protected areas, protective forests and vegetation, anthropic zones and flood zones) were established. Finally, the reality that the area presents particular conditions in terms of flood zones where both agricultural and aquaculture activities are carried out is highlighted, and that these must be considered and analyzed, especially within the 4,501.80 km2 that make up the “Prone” area (river overflow or heavy rainfall).