A selective fluorescent probe, MA12C, was developed specifically for the detection of polyvinyl chloride (PVC) microplastics. MA12C is an acridone derivative that presents fluorescence turn-on when binding to PVC microplastics through a twisted intramolecular charge transfer (TICT) mechanism. In this study, reaction condition between PVC and compound was evaluated in aqueous and in organic solvents with temperature dependent. Eventually, we concluded that higher incubated temperature (55 °C), with respect to room temperature, can promote interaction and increase the fluorescence-staining effect. Alternatively, these staining results seem to be solvent independent. Furthermore, the selectivity of MA12C to PVC was confirmed from microplastics polyethylene terephthalate (PET) and polystyrene (PS), which showed significantly weaker fluorescence. Hence, we constructed a fast-screening platform based on the fluorescence turn-on of MA12C when combined with a hand-held UV light and irradiated with 365 nm light source. Eventually, this platform was successfully applied to detect the PVC in soil with the naked eye. These findings indicate that MA12C is not only an effective tool for detecting PVC microplastics but also offers practical applications in environmental monitoring for human health, enabling rapid, low- cost, and selective detection of PVC in various contaminated matrices, contributing to the mitigation of plastic pollution.