Raman spectroscopy detects molecular signatures through elastic light scattering, and in recent years, it has gained popularity in sectors such as food, agriculture, forestry, fisheries, and animal husbandry. In this study, the authors developed a 3-dimensional plasmonic hotspot-rich (3D-PHS) nanostructure using randomly arranged silver nanowires in a woodpile formation. This nanochip features a random crossed-wire structure that generates unique 3D localized surface plasmon resonance (LSPR) and hotspots, enabling surface-enhanced Raman scattering (SERS). Compared to a 2D nanostructure, the 3D design increases the chip’s sensitivity by 1000 times, which not only reduces the sample amount required but also improves the detection limit. Consequently, the SERS spectra of Sudan Red dyes (I, II, III, IV) were effectively recorded and analyzed, achieving detection limits between 1 μM and 0.05 μM in a 20 μL sample. This 3D nanoplatform shows significant promise as an affordable, robust, and portable sensing device for potential future uses.