Scholarlink

Severe fever with thrombocytopenia syndrome (SFTS), an emerging tick-borne viral infection caused by the SFTS virus (SFTEV), poses a significant global public health threat due to its potential for pandemic spread. In this study, a self-assembly (Au-SH) method was used to sequentially attach 1,6-hexanedithiol (1,6-HDT) and colloidal gold nanoparticles to a gold film sputtered on a polyethylene terephthalate (PET) substrate to form an electrode with a single layer of gold nanoparticles. Then, the antigen of the SFTS viruses (SFTSV) are firmly attached to the electrode through the self-assembly method to make the biosensor for the detection of SFTSV antibody. The results of electrochemical impedance analysis show that the single-layer gold nanoparticle biosensor proposed in this research can successfully detect the antibody of SFTSV in 65 min with a wide linear detection range from 200 to 25000 ng/mL with a linearity of 0.9701. The detection limit is measured to be 2.076 ng/mL. The single-layer gold nanoparticle biosensor proposed in this research has the advantages of simple manufacturing process, low cost, high detection sensitivity, and short detection time. It has great commercial application feasibility.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Electrochemical Detection of the Emerging Pandemic Thrombocytopenia Syndrome (SFTS) Virus Antibodies

  • 会议论文

  • 发表日期：

    2025年

查看全文
  • Wei-Jhen Wang,
  • Ying-Ting Lin,
  • Yin-Ching Wu,
  • Gou-Jen Wang

摘要

Severe fever with thrombocytopenia syndrome (SFTS), an emerging tick-borne viral infection caused by the SFTS virus (SFTEV), poses a significant global public health threat due to its potential for pandemic spread. In this study, a self-assembly (Au-SH) method was used to sequentially attach 1,6-hexanedithiol (1,6-HDT) and colloidal gold nanoparticles to a gold film sputtered on a polyethylene terephthalate (PET) substrate to form an electrode with a single layer of gold nanoparticles. Then, the antigen of the SFTS viruses (SFTSV) are firmly attached to the electrode through the self-assembly method to make the biosensor for the detection of SFTSV antibody. The results of electrochemical impedance analysis show that the single-layer gold nanoparticle biosensor proposed in this research can successfully detect the antibody of SFTSV in 65 min with a wide linear detection range from 200 to 25000 ng/mL with a linearity of 0.9701. The detection limit is measured to be 2.076 ng/mL. The single-layer gold nanoparticle biosensor proposed in this research has the advantages of simple manufacturing process, low cost, high detection sensitivity, and short detection time. It has great commercial application feasibility.