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Hydroxyapatite (HA) is extensively utilized in bone tissue engineering due to its osteoconductive properties and high biocompatibility; however, its inherent brittleness and low degradation rate significantly limit its effectiveness in promoting cell proliferation and integration within host tissues. To address these drawbacks, this study investigated hydroxyapatite-incorporated silica aerogels (HA-SA-Np) with a HA/SiO2 ratio 0.5, synthesized from rice husk ash via an aqueous sol-gel process. The aim was to evaluate the efficacy of HA-SA-Np in the proliferation and adhesion of normal human fibroblast (HSF 1184) and osteoblast (NHOst) cells in vitro. This was done by comparing with free HA and silica aerogel nanoparticles (SA-Np) based on MTT assays, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) analysis after incubation periods of 3, 5, 7, and 21 days. The results demonstrated that HA-SA-Np significantly enhanced cell proliferation and adhesion, and HA-SA-Np significantly outperformed both HA and SA-Np in supporting cell growth. This was attributed to the controlled release of silicic acid and the consumption of phosphate ions, which are critical for cellular growth. These findings suggested that HA-SA-Np has strong potential as an advanced biomaterial for tissue engineering, offering enhanced support for cell attachment, proliferation, and tissue formation compared to conventional HA and silica-based material.

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Studies on the Growth of Human Cells on Hydroxyapatite Incorporated Silica Aerogel

  • 会议论文

  • 发表日期：

    2025年

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  • Nor Suriani Sani,
  • Nik Ahmad Nizam Nik Malek,
  • Ahmad Taufiq

摘要

Hydroxyapatite (HA) is extensively utilized in bone tissue engineering due to its osteoconductive properties and high biocompatibility; however, its inherent brittleness and low degradation rate significantly limit its effectiveness in promoting cell proliferation and integration within host tissues. To address these drawbacks, this study investigated hydroxyapatite-incorporated silica aerogels (HA-SA-Np) with a HA/SiO2 ratio 0.5, synthesized from rice husk ash via an aqueous sol-gel process. The aim was to evaluate the efficacy of HA-SA-Np in the proliferation and adhesion of normal human fibroblast (HSF 1184) and osteoblast (NHOst) cells in vitro. This was done by comparing with free HA and silica aerogel nanoparticles (SA-Np) based on MTT assays, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) analysis after incubation periods of 3, 5, 7, and 21 days. The results demonstrated that HA-SA-Np significantly enhanced cell proliferation and adhesion, and HA-SA-Np significantly outperformed both HA and SA-Np in supporting cell growth. This was attributed to the controlled release of silicic acid and the consumption of phosphate ions, which are critical for cellular growth. These findings suggested that HA-SA-Np has strong potential as an advanced biomaterial for tissue engineering, offering enhanced support for cell attachment, proliferation, and tissue formation compared to conventional HA and silica-based material.