Puma concolor (Linnaeus, 1771) is a large carnivorous mammal of the Felidae family, with a wide distribution across the Americas, inhabiting different environments. It was historically found in most of the territory in Argentina, but its distribution is currently restricted. The Pampas region has a scarce representation of pumas in archaeological contexts. Noteworthy, it is the archaeofaunal assemblage of the Las Marías site (Magdalena district, Buenos Aires province, Argentina), where 43 specimens have been recorded, with an estimated presence of four individuals. Evidence suggests human utilization of these animals based on the anthropic modifications observed. The aim of this study is to interpret the strategies related to the acquisition, use, and consumption of the P. concolor species at the Las Marías site and thus, to investigate the role they may have played in the sociocultural contexts of the prehispanic past of the Río de la Plata area. It is proposed that their use involved both domestic and symbolic experiences of the inhabitants of the site during the Late Holocene.