Food-borne pathogens pose significant health risks to both humans and animals worldwide. Health experts highlight that food safety is a critical global health issue, with the estimated total cost of detecting food-borne illnesses ranging from $5 to $6 billion. Salmonella is the most common pathogen responsible for food-borne illnesses, leading to salmonellosis and spreading daily through raw and processed foods, which presents a serious threat to public health. Detection methods for Salmonella, particularly in both developed and developing countries, are outdated. The primary detection methods are labor-intensive, costly, and can take several hours to days to yield results, often with a low detection limit. These methods are not suitable for analyzing large numbers of samples. Therefore, rapid detection of Salmonella is of great importance. Immunosensors present several advantages over traditional methods as they incorporate nanoparticles and micro- and nano-structured materials. Various immunosensors have been developed and commercialized for the detection of Salmonella. This chapter aims to provide a comprehensive overview of these immunosensors by highlighting the electrochemical signal-transducing mechanisms and analyzing recent trends, especially in conjunction with nanomaterials.