Biomaterials such as synthetic polymer and decellularized animal tissues have been widely studied for their biocompatibility, mechanical properties and 3-Dimensional shape in tissue engineering application. Due to potential cytotoxicity arises from usage of synthetic polymer to human cells and the cost of biodiversity impact when using animal tissue, alternative of these two sources is needed to find sustainable solution as biomaterial for scaffold application. This study focused on the decellularization of Mangiferaindica L. var Harumanis (HM) mango leaves using chloroform and sodium dodecyl sulfate (SDS) with 17% concentration. Observation of HM leaves were carried out every day for 6 days and analysis of its mechanical properties was completed using universal testing machine. From our analysis, young leaves of HM showed better potential for tissue engineering application as its showed tensile strength reading of 1.35Mpa and 1.48 Mpa. In conclusion, further studies are required in order to enhance the properties of HM leaves for clinical application in the future.