Virtual Reality (VR) applications hold significant promise for enhancing healthcare training by providing immersive and interactive environments for skills development. This scoping review analyzed the methods, and instruments used for usability assessment in VR applications designed to support healthcare training. An electronic database search identified 19 studies meeting specific inclusion and exclusion criteria. The included studies focused on two primary objectives: (i) usability assessment, and (ii) usability and feasibility assessment, evaluating not only the usability but also the practicality and sustainability of VR applications in healthcare training settings. The findings reveal inconsistencies in the reporting of methodological details essential for robust usability assessment, particularly in terms of methods’ triangulation and participants’ sample sizes. This review highlights the need for more rigorous and comprehensive approaches that combine both test and inquiry methods to ensure that VR applications present good usability, which is impactful for sustainable healthcare training applications.