As an important part of the core competitiveness of enterprises, the shaping and influence of corporate culture on employee behavior cannot be ignored. Employee behavior, as the external embodiment of enterprise culture, is directly related to the operational efficiency and market competitiveness of the enterprise. Corporate culture not only covers the values, beliefs, customs and practices of the enterprise, but is also reflected in the daily work behavior and decision-making of employees. A positive and healthy corporate culture can stimulate the spirit of innovation and teamwork among employees, thus improving their job satisfaction and loyalty. This paper delves into the correlation between corporate culture and employee behavior, and proposes a deep learning-based prediction model to quantify and predict this relationship. In this paper, a deep learning-based prediction model is constructed for quantifying and predicting the correlation between corporate culture and employee behavior. The results of the model can not only provide enterprises with profound insights about the relationship between corporate culture and employee behavior, but also provide a reference basis for them to formulate more accurate human resource management strategies. The deep learning-based prediction model provides a new, quantitative analysis tool for enterprises.