Extracts from the leaves of plants of the genus Verbesina have shown promising biomedical potential, especially for their antimicrobial and anti-inflammatory properties and their application in wound healing. In this study, we analyzed the biological properties of the methanolic extract of Verbesina persicifolia using the minimum inhibitory concentration (MIC) assay to determine its antimicrobial activity against various pathogenic bacteria. In addition, cell viability was evaluated using the Alamar Blue assay to determine the effect of plant extracts on bacterial viability and the cytotoxicity of the extracts in murine fibroblasts in culture. The results demonstrated significant antimicrobial activity, making the extract a potential candidate for the development of new antimicrobial agents. Furthermore, the non-toxic effects on fibroblasts highlight its potential to promote wound healing, suggesting that Verbesina persicifolia could serve as a natural alternative for therapeutic applications in wound management.