Oxidized titanium alloys, such as Ti 6Al 4V, are widely used in medical implants because their biocompatibility depends on surface properties like morphology, chemistry, and electrical charge. Laser processing in atmospheric, oxygenated conditions produces a thin oxide film with an embedded electrical charge, altering these surface characteristics. Although SEM is routinely employed to assess morphology and chemistry, its potential for evaluating surface charge is underexplored. In this study, we laser processed Ti 6Al 4V samples and used SEM in backscattered electron (BSE) mode to quantify surface charging. We correlated changes in BSE signal strength with work function measurements, finding that a work function increase from approximately 4.85 to 5.00 eV—indicative of greater surface charging—was accompanied by a measurable decrease in BSE image grey levels. Measurements at 10, 15, and 20 keV revealed a significant difference between 10 and 15 keV, while at 20 keV, the effect of surface charge on electron scattering was minimal. This approach demonstrates that BSE imaging can integrate charge analysis into routine SEM evaluations of laser-processed implant surfaces.