This chapter explores the multifaceted role of platelets in regenerative medicine, with a primary focus on autologous use of platelets commonly named platelet-rich plasma (PRP). Originating from a supraphysiological concentration of platelets, PRP is a potent source of growth factors and bioactive molecules that stimulate tissue repair and regeneration. This section reviews the evolution of use of autologous PRP, from its first application in maxillofacial surgery to its use across diverse clinical fields such as musculoskeletal disorders, osteoarthritis, wound healing, and alopecia. Key challenges include the heterogeneity of preparation methods, variability in clinical outcomes, and the need for standardized protocols. The chapter also examines emerging alternatives, such as allogeneic platelet products and platelet lysates, which address limitations of autologous PRP by offering scalability and broader applicability. Advances in platelet-derived extracellular vesicles further highlight innovative approaches to targeted drug delivery and tissue engineering. These developments emphasize the transformative potential of platelets in regenerative medicine and beyond.