This chapter discusses sustainable materials derived from lignin for tissue engineering. Lignin is a complex, sustainable biopolymer that is abundantly available, making it an attractive alternative to traditional synthetic materials. Currently, lignin is used for low-value manufacturing, such as energy production by incineration. Lignin has emerged to provide unique mechanical and chemical properties for tissue engineering applications. Its antioxidant, antimicrobial, biodegradability, biocompatibility, and mechanical strength are of great interest and are discussed here. Lignin has excellent potential for biomedical engineering. However, further research and studies on appropriate animal models are needed to verify lignin-derived biomaterials as a safe and viable option for tissue engineering applications, particularly its long-term stability. Developing lignin-derived materials for tissue engineering can address some of the challenges associated with traditional biomaterials, including cost, availability, and sustainability.