Sustainable scaffolds have become crucial in tissue engineering and repair as well as regenerative steam cell therapy, offering eco-friendly solutions for tissue repair and regeneration. Despite advancements in biomaterials, current technologies still face challenges in achieving desired stability and efficiency in tissue defect repair. The advancement in regenerative tissue engineering and scaffold synthesis is evolving toward the development of durable scaffolds that provide structural support for tissue, promoting healing while minimizing waste through sustainable practices. The global sustainable scaffold market is projected to reach €7 billion by 2025, with a 15% annual growth rate. The key innovations highlighted include the synthesis of three-dimensional porous and hydrogel scaffolds that mimic the synthesis of extracellular matrix using natural vibers that enable cell infiltration and tissue regeneration. The porosity, pore size, and interconnectivity of scaffolds are critical for effective cell growth. Green manufacturing processes, such as the use of biodegradable materials (chitosan, cellulose, alginate), solvent-free methods (freeze drying, electrospinning, supercritical CO2 foaming), and 3D printing, enhance sustainability by minimizing energy consumption, chemical waste, and environmental impact. These processes improve biocompatibility while reducing material waste and carbon footprints. Overall, sustainable scaffold fabrication supports regenerative medicine’s goals while aligning with broader environmental sustainability objectives, making it a key area for future biomedical advances.