The objective of this work is to investigate the effect of glutaraldehyde crosslinking on the properties of electrospun gelatin. Using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC) techniques, it was observed how the chemical crosslinking process with glutaraldehyde (GTA) modifies the properties of electrospun gelatin. FTIR analysis demonstrated the pre-existence of glutaraldehyde residues in the electrospun gelatin, confirming the formation of cross-links between gelatin molecules. SEM images showed that crosslinking with glutaraldehyde resulted in an increase in the diameter of the electrospun gelatin fibers, indicating a higher degree of crosslinking and a denser structure. DSC tests revealed an increase in the denaturation temperature of the crosslinked electrospun gelatin. This suggests that glutaraldehyde crosslinking strengthens the gelatin structure, making it more resistant to thermal denaturation. The study demonstrates that glutaraldehyde crosslinking is an effective technique for modifying the properties of electrospun gelatin. This method increases the thermal and mechanical stability of gelatin, making it a stronger and more versatile material for various biomedical and tissue engineering applications. The findings of this work have important implications for the development of gelatin-based biomaterials with improved properties.