The use of composite materials, especially carbon fiber, has provided a good cost-benefit ratio for civil structures, especially to long term, as it allows for the reduction of maintenance operations. This aspect is especially important when dealing with environments with high levels of aggressive agents. Carbon fibers were originally used in civil engineering as reinforcement for connections, aiming to adapt the structural response to seismic actions. In principle, the use of carbon fiber increases the stiffness of the connections without significantly increasing mass. An alternative technique, currently under study, is the use of carbon fiber in connections between wooden truss bars, in order to dispense with the use of metal connectors. For this purpose, experimental tests were carried out on triangular prototypes to find an average value of rotational stiffness of the connection, which accounts for the use of carbon fiber with epoxy resin. In a later step, this parameter is introduced into a computational model for analyzing the natural vibration frequencies and modes. The model represents a roof truss designed to overcome a free span of 16 m in length. In the modal analysis, an increase in the frequency of the first mode of around 0.43% and 15% for the seventh mode could be observed, when compared to the traditional calculation method, which considers the hinged connection. If the structure's own weight is computed in the analysis, these differences correspond to 0.77% and 20%, respectively.