This study investigates the dynamic behavior of an innovative hybrid timber-concrete composite (TCC) floor system with concrete slab supports designed to simplify assembly and enhance efficiency. A full-scale TCC floor was tested to evaluate its dynamic properties and vibration performance under human-induced excitation. The modal analysis determined natural frequencies and mode shapes, while walking tests assessed acceleration levels at different step frequencies. The fundamental frequency of 9.76 Hz satisfies design thresholds, but vibration levels during walking exceeded comfort limits for residential and office environments, as defined by ISO standards. These findings highlight the limitations of traditional frequency-based criteria and underscore the importance of detailed dynamic assessments. This research advances the understanding of the vibration performance of TCC floors as sustainable and efficient structural systems.